ProGP1225 (FlaB3)
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| ProGP ID | ProGP1225 (FlaB3) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Methanothermococcus thermolithotrophicus |
| Domain | Archaea |
| Classification | Phylum : Euryarchaeota Class : Methanococci Orders : Methanococcales Family : Methanococcaceae Genus : Methanococcus Species : thermolithotrophicus |
| Taxonomic ID (NCBI) | 2186 |
| Genome Information | |
| GenBank | AF333250 |
| EMBL | AF333250.1 |
| Organism Additional Information | It is a thermophilic methanogen |
| Gene Information | |
| Gene Name | flaB3 |
| Protein Information | |
| Protein Name | archaellum |
| UniProtKB/SwissProt ID | Q9C4Q0 |
| NCBI RefSeq | AF333250_3 |
| UniProtKB Sequence | >tr|Q9C4Q0|Q9C4Q0_METTL Flagellin OS=Methanothermococcus thermolithotrophicus OX=2186 GN=flaB3 PE=3 SV=1 MKIFEFLKNKKGASGIGTLIVFIAMVLVAAVAASVLINTSGFLQQKASSTGKESTEQVAS GLQTLQVLGMHNNANINYTAIYISPNAGSAPIDLEQAVVMMSDGATKLVAKYNDTSNDYD SLTSGGSVFESDVWNSVKNTEFGIIVIQDADGSCKNTTPVINKGDIVAIALNTTGLSMDP RSEITGTITPEFGAPAIISFTTPATYLDDSRIIQLQ |
| Subcellular Location | Archaellum |
| Function | Motility and stability |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N156 |
| Glycosite(s) Annotated Protein Sequence | NTTPVINK |
| Technique(s) used for Glycosylation Detection | SDS-PAGE, Western blotting, nano-LC-MS/MS, HILIC-MS |
| Glycan Information | |
| Glycan Annotation | 3-O-methyl-5-C-methyl-4-O-acetyl-β-galactopyranosyluronic acid, α-DDHep3,6OMe2, α-GalNAcA3OMe, α-GalA, α-GalAN, β-Man, β-Man |
| Technique(s) used for Glycan Identification | RP LC-MS/MS, HILIC-MS/MS, NMR analysis |
| Literature | |
| Year of Identification | 2020 |
| Reference | Kelly, J.F., Vinogradov, E., Stupak, J., Robotham, A.C., Logan, S.M., Berezuk, A., Khursigara, C.M. and Jarrell, K.F., 2020. Identification of a novel N-linked glycan on the archaellins and S-layer protein of the thermophilic methanogen, Methanothermococcus thermolithotrophicus. Journal of Biological Chemistry, 295(43), pp.14618-14629. |